Increasing temperature generally causes fluorescence intensity to:

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Increasing temperature typically causes fluorescence intensity to decrease due to several factors related to the behavior of fluorescent molecules. As temperature rises, the molecules gain more kinetic energy, which can lead to increased molecular motion. This enhanced motion may result in phenomena such as non-radiative energy dissipation, where excitation energy is released as heat rather than as emitted light. Additionally, higher temperatures can lead to increased molecular collisions and vibrations within the sample, which can quench the fluorescence signal and reduce overall fluorescence intensity.

In certain cases, the increase in temperature may also change the environment around the fluorescent molecules, such as altering the pH or the polarity of the solvent, which in turn can further impact the efficiency of fluorescence. Therefore, the general trend observed in many fluorescent systems is a decrease in fluorescence intensity with increasing temperature, making the answer to the question correct.

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